Particulate Organic Carbon and Nitrogen Concentrations in Lakes


An important part of the McMurdo Long Term Ecological Research (LTER) is evaluating carbon and nitrogen budgets in perennial ice-covered lakes. This dataset addresses this core area of research and quantifies the particulate carbon and nitrogen found at specific depths in McMurdo Dry Valley lakes.

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Lake water samples were collected at specific depths with a five-liter Niskin bottle during normal LTER limnological sampling. Sub-samples were decanted into three-1 L Nalgene bottles (2-light and 1-amber), two-500 mL amber Nalgene bottles, three-150 mL borosilicate glass bottles, two-20 mL scintillation vials, and one-30 mL serum vial. A 500 mL sample from the one liter amber Nalgene bottle was decanted and filtered through a combusted Whatman 25 mm GF/F filter (475 degrees C for 4 hours). The filter was placed onto an aluminum weighing pan and air dried. Once the filters were dry, the pans were stacked together and frozen for later analysis. 

Filters were acidified with HCL fumes, and analyzed using an Elemental Analyzer (currently a CE Elantech Flash EA 1112 Elemental Analyzer). Total particulate organic carbon (POC) and particulate organic nitrogen (PON) was obtained by comparison with an acetanilide standard curve.

Prior to the 0304 season, analysis was done on a 10 mm diameter sub-sample punch of the GF/F filter; from 0304 to present, the entire GF/F filter was analyzed.

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Data from this table was submitted to INSTAAR by John Priscu's team at Montana State University. The raw data files listed under 'file name' are the names of the original files submitted. The 1993/94 and 1994/95 datasets are Microsoft Excel version 6.0 files, and the 1995/96, 1996/97 and 1997/98 datasets are ascii text files. Upon arrival at INSTAAR, the data manager fine-tuned the location codes and limno runs to match those provided in the "locations, dates, codes for lake chemistry, biology samples" file. The file was imported into Microsoft Access on INSTAAR's Unix system, and can currently be found there. The file  was then exported in ascii, comma delimited text and MS-DOS text (table layout) to present on the MCM LTER web site. Both of these files are linked to this web page above. Information for the metadata was obtained from the Metachn9697.rtf file. The file was called up using  Microsoft Word version 6.0. Text from this file was used to create this page in html format.
 Data for the 2005-6 and 2006-7 season was updated in Oct 2009
 Metadata was standardized to EML in 2006, and enhanced in 2014 using the Drupal Ecological Information Management System

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Instrument detection limits for POC and PON are 10 ug punch -1, and 5 ug punch -1, respectively.


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